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Record #200008:

Improved direct-count method by fluorescence microscope for low-biomass ice core (Japanese) / Jun Uetake, Kumiko Goto-Azuma, Hideaki Motoyama.

Title: Improved direct-count method by fluorescence microscope for low-biomass ice core (Japanese) / Jun Uetake, Kumiko Goto-Azuma, Hideaki Motoyama.
Author(s): Uetake, Jun.
Goto-Azuma, Kumiko.
Motoyama, Hideaki.
Date: 2012.
In: Antarctic Record (NIPR) = Nankyoku Shiryō. (2012.), Vol. 56(1) (2012)
Abstract: Hypothesises that low microorganism count in ice cores as measured by direct cell count under fluorescence microscope is underestimation due to degradation of fluorescent dye or because non-specific fluorescence from mineral particles inhibits fluorescence signal from microorganisms. Describes test of four types of anti-fade solution and 19 types of fluorescent dye to select best combination in terms of prolonging fluorescent signal from microorganisms and distinguishing biological signals from noise produced by non-specific particles. Finds optimal combination is nuclear acid dye "YOYO-1" (Molecular ProbesR) and antifade solution "Everbrite Mounting Medium" (Biotium)
Notes:

Antarctic Record (NIPR) = Nankyoku Shiryō. Vol. 56(1) :57-67 (2012).

Keywords: 551.32 -- Glaciology.
551.324 -- Land ice.
622.14 -- Core sampling, ice and snow.
57 -- Biology.
579 -- Microbiology and bacteriology.
54 -- Chemistry.
E14 -- Glaciology: biological aspects of ice and snow.
SPRI record no.: 200008

MARCXML

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007 ta
008 220930s2012####xx#a##|##|###|0||#0|eng#d
035 ## ‡aSPRI-200008
040 ## ‡aUkCU-P‡beng‡eaacr
100 1# ‡aUetake, Jun.
245 10 ‡aImproved direct-count method by fluorescence microscope for low-biomass ice core (Japanese) /‡cJun Uetake, Kumiko Goto-Azuma, Hideaki Motoyama.
260 ## ‡a[S.l.] :‡b[s.n.],‡c2012.
300 ## ‡ap. 57-67 :‡bill., diags., tables.
500 ## ‡aAntarctic Record (NIPR) = Nankyoku Shiryō. Vol. 56(1) :57-67 (2012).
520 3# ‡aHypothesises that low microorganism count in ice cores as measured by direct cell count under fluorescence microscope is underestimation due to degradation of fluorescent dye or because non-specific fluorescence from mineral particles inhibits fluorescence signal from microorganisms. Describes test of four types of anti-fade solution and 19 types of fluorescent dye to select best combination in terms of prolonging fluorescent signal from microorganisms and distinguishing biological signals from noise produced by non-specific particles. Finds optimal combination is nuclear acid dye "YOYO-1" (Molecular ProbesR) and antifade solution "Everbrite Mounting Medium" (Biotium)
650 07 ‡a551.32 -- Glaciology.‡2udc
650 07 ‡a551.324 -- Land ice.‡2udc
650 07 ‡a622.14 -- Core sampling, ice and snow.‡2udc
650 07 ‡a57 -- Biology.‡2udc
650 07 ‡a579 -- Microbiology and bacteriology.‡2udc
650 07 ‡a54 -- Chemistry.‡2udc
650 07 ‡aE14 -- Glaciology: biological aspects of ice and snow.‡2local
700 1# ‡aGoto-Azuma, Kumiko.
700 1# ‡aMotoyama, Hideaki.
773 0# ‡7nnas ‡tAntarctic Record (NIPR) = Nankyoku Shiryō. ‡gVol. 56(1) (2012) ‡wSPRI-29187
916 ## ‡a2012/07/25 -- JHR
917 ## ‡aUnenhanced record from Muscat, imported 2019
948 3# ‡a20220930 ‡bJHR