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Record #200008:
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MARC recordImproved direct-count method by fluorescence microscope for low-biomass ice core (Japanese) / Jun Uetake, Kumiko Goto-Azuma, Hideaki Motoyama.
| Title: | Improved direct-count method by fluorescence microscope for low-biomass ice core (Japanese) / Jun Uetake, Kumiko Goto-Azuma, Hideaki Motoyama. |
| Author(s): | Uetake, Jun. Goto-Azuma, Kumiko. Motoyama, Hideaki. |
| Date: | 2012. |
| In: | Antarctic Record (NIPR) = Nankyoku Shiryō. (2012.), Vol. 56(1) (2012) |
| Abstract: | Hypothesises that low microorganism count in ice cores as measured by direct cell count under fluorescence microscope is underestimation due to degradation of fluorescent dye or because non-specific fluorescence from mineral particles inhibits fluorescence signal from microorganisms. Describes test of four types of anti-fade solution and 19 types of fluorescent dye to select best combination in terms of prolonging fluorescent signal from microorganisms and distinguishing biological signals from noise produced by non-specific particles. Finds optimal combination is nuclear acid dye "YOYO-1" (Molecular ProbesR) and antifade solution "Everbrite Mounting Medium" (Biotium) |
| Notes: | Antarctic Record (NIPR) = Nankyoku Shiryō. Vol. 56(1) :57-67 (2012). |
| Keywords: | 551.32 -- Glaciology. 551.324 -- Land ice. 622.14 -- Core sampling, ice and snow. 57 -- Biology. 579 -- Microbiology and bacteriology. 54 -- Chemistry. E14 -- Glaciology: biological aspects of ice and snow. |
| SPRI record no.: | 200008 |
LDR 01742naa#a2200000#a#4500 001 SPRI-200008 005 20230923162753.0 007 ta 008 230923s2012####xx#a##|##|###|0||#0|eng#d 035 ## ‡aSPRI-200008 040 ## ‡aUkCU-P‡beng‡eaacr 100 1# ‡aUetake, Jun. 245 10 ‡aImproved direct-count method by fluorescence microscope for low-biomass ice core (Japanese) /‡cJun Uetake, Kumiko Goto-Azuma, Hideaki Motoyama. 260 ## ‡a[S.l.] :‡b[s.n.],‡c2012. 300 ## ‡ap. 57-67 :‡bill., diags., tables. 500 ## ‡aAntarctic Record (NIPR) = Nankyoku Shiryō. Vol. 56(1) :57-67 (2012). 520 3# ‡aHypothesises that low microorganism count in ice cores as measured by direct cell count under fluorescence microscope is underestimation due to degradation of fluorescent dye or because non-specific fluorescence from mineral particles inhibits fluorescence signal from microorganisms. Describes test of four types of anti-fade solution and 19 types of fluorescent dye to select best combination in terms of prolonging fluorescent signal from microorganisms and distinguishing biological signals from noise produced by non-specific particles. Finds optimal combination is nuclear acid dye "YOYO-1" (Molecular ProbesR) and antifade solution "Everbrite Mounting Medium" (Biotium) 650 07 ‡a551.32 -- Glaciology.‡2udc 650 07 ‡a551.324 -- Land ice.‡2udc 650 07 ‡a622.14 -- Core sampling, ice and snow.‡2udc 650 07 ‡a57 -- Biology.‡2udc 650 07 ‡a579 -- Microbiology and bacteriology.‡2udc 650 07 ‡a54 -- Chemistry.‡2udc 650 07 ‡aE14 -- Glaciology: biological aspects of ice and snow.‡2local 700 1# ‡aGoto-Azuma, Kumiko. 700 1# ‡aMotoyama, Hideaki. 773 0# ‡7nnas ‡tAntarctic Record (NIPR) = Nankyoku Shiryō. ‡gVol. 56(1) (2012) ‡wSPRI-29187 916 ## ‡a2012/07/25 -- JHR 917 ## ‡aUnenhanced record from Muscat, imported 2019 948 3# ‡a20230923 ‡bJHR