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Record #186441:
Construction of a low-temperature protein expression system using a cold-adapted bacterium, Shewanella sp. strain Ac10, as the host / Ryoma Miyake, and 6 others.
Title: | Construction of a low-temperature protein expression system using a cold-adapted bacterium, Shewanella sp. strain Ac10, as the host / Ryoma Miyake, and 6 others. |
Author(s): | Miyake, Ryoma. |
Date: | 2007. |
Publisher: | [Washington, DC]: American Society for Microbiology |
Abstract: | Recombinant protein expression systems working at low temperatures are expected to be useful for production of thermolabile proteins. Evaluation of promoters for proteins abundantly produced at 4°C in Shewanella sp. strain Ac10, isolated from Antarctic seawater, to express foreign proteins indicates that amounts of putative peptidases PepF and PepQ produced by this system were greater than those produced by Escherichia coli T7 promoter system. |
Notes: | Offprint: Applied and Environmental Microbiology. Vol. 73, no. 15. |
Keywords: | 574 -- Ecology. 577.1 -- Biochemistry. 579 -- Microbiology and bacteriology. F -- Biological sciences. (*7) -- Antarctic regions. (*80) -- Southern Ocean. |
Location(s): | SCO: SPRI-PAM: (*7) : 579[M] |
SPRI record no.: | 186441 |
LDR 01577nam#a2200000#a#4500 001 SPRI-186441 005 20240329132451.0 007 ta 008 240329s2007####dcu####|#2###|0||#0#eng#d 035 ## ‡aSPRI-186441 040 ## ‡aUkCU-P‡beng‡eaacr 100 1# ‡aMiyake, Ryoma. 245 10 ‡aConstruction of a low-temperature protein expression system using a cold-adapted bacterium, Shewanella sp. strain Ac10, as the host /‡cRyoma Miyake, and 6 others. 260 ## ‡a[Washington, DC] :‡bAmerican Society for Microbiology,‡c2007. 300 ## ‡ap. 4849-4856 ;‡c30 cm. 490 0# ‡aApplied and Environmental Microbiology 500 ## ‡aOffprint: Applied and Environmental Microbiology. Vol. 73, no. 15. 520 3# ‡aRecombinant protein expression systems working at low temperatures are expected to be useful for production of thermolabile proteins. Evaluation of promoters for proteins abundantly produced at 4°C in Shewanella sp. strain Ac10, isolated from Antarctic seawater, to express foreign proteins indicates that amounts of putative peptidases PepF and PepQ produced by this system were greater than those produced by Escherichia coli T7 promoter system. 530 ## ‡aAlso issued online ‡uurn:doi:10.1128/AEM.00824-07‡uhttps://dx.doi.org/10.1128/AEM.00824-07 650 07 ‡a574 -- Ecology.‡2udc 650 07 ‡a577.1 -- Biochemistry.‡2udc 650 07 ‡a579 -- Microbiology and bacteriology.‡2udc 650 07 ‡aF -- Biological sciences.‡2local 651 #7 ‡a(*7) -- Antarctic regions.‡2udc 651 #7 ‡a(*80) -- Southern Ocean.‡2udc 852 7# ‡2camdept‡bSCO‡cSPRI-PAM‡h(*7) : 579[M]‡9Create 1 item record‡0Migrate 916 ## ‡a139641 -- 2009/02/19 -- HS 917 ## ‡aUnenhanced record from Muscat, imported 2019 948 3# ‡a20240329 ‡bHS