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Record #173411:

Recent molecular biological studies of Vostok ice core, Antarctica / S.A. Bulat, I.A. Alekhina, J.R. Petit, D. Marie, V.Ya. Lipenkov, V.V. Lukin.

Title: Recent molecular biological studies of Vostok ice core, Antarctica / S.A. Bulat, I.A. Alekhina, J.R. Petit, D. Marie, V.Ya. Lipenkov, V.V. Lukin.
Author(s): Bulat, S. A.
Alekhina, I. A.
Petit, J. R.
Marie, D.
Lipenkov, V. Ya.
Lukin, V. V.
Date: 2004.
Language: Russian transliteration entered into original records using BGN/PCGN 1947 romanisation of Russian; Cyrillic text in MARC 880 field(s) reverse transliterated from this by automated process; BGN/PCGN 1947 text then upgraded to Library of Congress romanisation.
In: Materialy Gli︠a︡t︠s︡iologicheskikh Issledovaniĭ. (2004.), Vol. 97 (2004)
Abstract: Summarizes study of microbial contents of accretion ice from subglacial lake Vostok, including PCR based bacterial and archaeal gene sequencing, epifluorescent and confocal microscopies and flow cytometry. Argues microbes found in uppermost (only) of two accretion ice layers examined would not survive highly oxygenated lake environment, but could live in lake bottom sediments and be entrapped in accretion ice. Of glacial ice, only basal layer yielded (two) phylotypes, apparently representing bedrock, rather than lake, life. Flow cytometry estimates of 9 and 24 cells/ml for accretion and deep glacial ice and 10:1 contaminant to indigenous cells ratio (PCR results) suggest genuine microbial contents may be only 1 cell/ml. Discusses lessons for search for extraterrestrial life.
Notes:

Materialy Gli︠a︡t︠s︡iologicheskikh Issledovaniĭ. Vol. 97 :89-90 (2004).

Keywords: 551.32 -- Glaciology.
57 -- Biology.
579 -- Microbiology and bacteriology.
575 -- Genetics.
57.08 -- Biological techniques and methods.
551.321.2 -- Snow and ice, methods of sampling, excavating, drilling, etc.
622.233 -- Drills and drilling, ice and snow sampling.
551.324 -- Land ice.
551.324.24 -- Ice sheets and caps.
551.332.56 -- Lakes, ice-dammed. Glacial lakes. Disruption of water drainage: Vostok.
574.23 -- Habitat conditions, extreme.
574.5 -- Marine and freshwater ecology.
E14 -- Glaciology: biological aspects of ice and snow.
(*7) -- Antarctic regions.
(*747.5) -- Vostok region.
SPRI record no.: 173411

MARCXML

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005 20221004190233.0
007 ta
008 221004s2004####xx####|##|###|0||#0|eng#d
035 ## ‡aSPRI-173411
040 ## ‡aUkCU-P‡beng‡eaacr
245 00 ‡aRecent molecular biological studies of Vostok ice core, Antarctica /‡cS.A. Bulat, I.A. Alekhina, J.R. Petit, D. Marie, V.Ya. Lipenkov, V.V. Lukin.
260 ## ‡a[S.l.] :‡b[s.n.],‡c2004.
300 ## ‡ap. 89-90.
500 ## ‡aMaterialy Gli︠a︡t︠s︡iologicheskikh Issledovaniĭ. Vol. 97 :89-90 (2004).
520 3# ‡aSummarizes study of microbial contents of accretion ice from subglacial lake Vostok, including PCR based bacterial and archaeal gene sequencing, epifluorescent and confocal microscopies and flow cytometry. Argues microbes found in uppermost (only) of two accretion ice layers examined would not survive highly oxygenated lake environment, but could live in lake bottom sediments and be entrapped in accretion ice. Of glacial ice, only basal layer yielded (two) phylotypes, apparently representing bedrock, rather than lake, life. Flow cytometry estimates of 9 and 24 cells/ml for accretion and deep glacial ice and 10:1 contaminant to indigenous cells ratio (PCR results) suggest genuine microbial contents may be only 1 cell/ml. Discusses lessons for search for extraterrestrial life.
546 ## ‡aRussian transliteration entered into original records using BGN/PCGN 1947 romanisation of Russian; Cyrillic text in MARC 880 field(s) reverse transliterated from this by automated process; BGN/PCGN 1947 text then upgraded to Library of Congress romanisation.
650 07 ‡a551.32 -- Glaciology.‡2udc
650 07 ‡a57 -- Biology.‡2udc
650 07 ‡a579 -- Microbiology and bacteriology.‡2udc
650 07 ‡a575 -- Genetics.‡2udc
650 07 ‡a57.08 -- Biological techniques and methods.‡2udc
650 07 ‡a551.321.2 -- Snow and ice, methods of sampling, excavating, drilling, etc.‡2udc
650 07 ‡a622.233 -- Drills and drilling, ice and snow sampling.‡2udc
650 07 ‡a551.324 -- Land ice.‡2udc
650 07 ‡a551.324.24 -- Ice sheets and caps.‡2udc
650 07 ‡a551.332.56 -- Lakes, ice-dammed. Glacial lakes. Disruption of water drainage: Vostok.‡2udc
650 07 ‡a574.23 -- Habitat conditions, extreme.‡2udc
650 07 ‡a574.5 -- Marine and freshwater ecology.‡2udc
650 07 ‡aE14 -- Glaciology: biological aspects of ice and snow.‡2local
651 #7 ‡a(*7) -- Antarctic regions.‡2udc
651 #7 ‡a(*747.5) -- Vostok region.‡2udc
700 1# ‡aBulat, S. A.
700 1# ‡aAlekhina, I. A.
700 1# ‡aPetit, J. R.
700 1# ‡aMarie, D.
700 1# ‡aLipenkov, V. Ya.
700 1# ‡aLukin, V. V.
773 0# ‡6880-01‡7nnas ‡tMaterialy Gli︠a︡t︠s︡iologicheskikh Issledovaniĭ. ‡gVol. 97 (2004) ‡wSPRI-33585
880 0# ‡6773-01/(N‡7nnas ‡tМатериалы Гляциологических Исследований. ‡gVol. 97 (2004) ‡wSPRI-33585
916 ## ‡a2005/10/18 -- RF
917 ## ‡aUnenhanced record from Muscat, imported 2019
948 3# ‡a20221004 ‡bRF